Stekas B, Yeo S, Troitskaia A, Honda M, Sho S, Spies M, Chemla YR.(2021) "Optical tweezers in single-molecule biophysics" Nature Reviews Methods Primers 1:25 Bustamante CJ, Chemla YR, Liu S, Wang MD.Ha, eds.) Cold Spring Harbor Laboratory Press, Woodbury NY.
WHERE IS I LEAP OFFICE AT UIUC MANUAL
In Single Molecule Techniques: A Laboratory Manual (P. High resolution dual trap optical tweezers with differential detection. Rossman, eds.) Springer, New York, NY (2011) Chemla, Y.R., Smith, D.E., Single-molecule studies of viral DNA packaging.(2016) "High-Resolution Fleezers: Dual-trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection" in Optical Tweezers (Arne Gennerich Ed.), Springer. Whitley K.D., Comstock M.J., Chemla Y.R.In Single-molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods, Methods in Enzymology, Vol. (2017) High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy. Interested students and postdocs with backgrounds in physics, biology, chemistry, or related fields are welcome to contact me. Students in my laboratory will work on all facets of research in this area: design and construction of instrumentation, development of biological systems for single-molecule manipulation, and quantitative analysis and modeling of collected data. Access to this length scale should lead to a more detailed and refined understanding of many fundamental processes. These high-resolution optical trapping techniques have the potential to reveal, for the first time, the stepwise motions of a host of molecular motors that translocate along or interact with nucleic acids and proteins. Recently, advances to this technique have made it possible to resolve motions on the scale of a single base pair of DNA, or only 3.4Å (see for example, Moffitt et al., PNAS, 2006). Optical traps, or “optical tweezers,” which utilize the force generated by focused laser light to manipulate microscopic objects, have been used extensively to measure the movements and forces exerted by individual molecular motors. A broad area of interest in my laboratory will be understanding the mechanism by which these molecular machines operate, and specifically, the process of mechano-chemical conversion.īiophysical techniques that can detect such processes at the level of a single molecule are extremely powerful, since they are not subject to the averaging artifacts of traditional bulk biochemical methods. Molecular motors, as they are called, are involved in such diverse processes as replicating the genome or transporting cargo across the cell, typically moving in discrete steps along a track - actin, microtubules, or DNA itself - converting chemical energy into mechanical work. The cell is a factory of complex molecular structures that carry out specialized mechanical tasks and that behave remarkably like machines. He joined the Department of Physics at Illinois in January 2007. In 2005, he received one of the prestigious Career Awards at the Scientific Interface (CASI) from the Burroughs-Wellcome Fund. There, he learned the techniques of single-molecule manipulation and used an optical trap to study viral DNA packaging (Chemla et al., Cell, 2005). Carlos Bustamante’s laboratory at Berkeley. Chemla made the “leap” to biophysics as a postdoctoral fellow, moving down the hall to Prof. As an experimentalist in applied superconductivity, he developed an interest in biology through his study of magnetotactic bacteria with a superconducting magnetometer (Chemla et al., Biophys J., 1999), and the development of a biosensor based on functionalized magnetic nanoparticles (Chemla et al., PNAS, 2000). in physics from the University of California, Berkeley in 2001. Ph.D., Physics, University of California-Berkeley, 2001.This statement was issued on Augby Entrata, Inc.229 Loomis Laboratory For more information We will do our best to respond to your feedback within five business days. Feedback:Įntrata welcomes feedback in relation to the accessibility of this website. Entrata is proud of the efforts that we have completed and that are in-progress to ensure that our website is accessible to everyone. The World Wide Web Consortium (W3C) has established Web Content Accessibility Guidelines (WCAG) for website designers and developers to improve accessibility for individuals with disabilities, and this website strives to be conformant to WCAG 2.1 level AA. We are continually applying relevant accessibility standards to improve user experience for everyone who visits this website. (“Entrata”) is dedicated to ensuring digital accessibility for people with disabilities.
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